Study on extraction process of alcohol-soluble ingredients from Hellgrammites(Megaloptera: Corydalidae)

Yuyun Chen; Ming Pan; Daifang Zhang

doi:10.23977/erej.2018.21002

Study on extraction process of alcohol-soluble ingredients from Hellgrammites(Megaloptera: Corydalidae)

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DOI: 10.23977/erej.2018.21002 | Downloads: 42 | Views: 4484

Author(s)

Daifang Zhang ¹, Ming Pan ², Yuyun Chen ¹

Affiliation(s)

¹ Department of Chemical Engineering, Sichuan University of Science & Engineering, Zigong643000, Sichuan, China
² School of Bioengineering, Sichuan University of Science & Engineering, Zigong643000, Sichuan, China

Corresponding Author

Yuyun Chen

ABSTRACT

Study the alcohol soluble ingredient extraction process of Hellgrammites and the significance of whether it can be exploited the medicinal liquor. Using the crude product of Hellgrammites, the impact factors are the ethanol concentration, the extracting time and the ratio of the material liquid. These three factors are in the three level to do the orthogonal experiments. Using the alcohol extract of Hellgrammites as the sample, the meteorological mass spectrometry and the inductively coupled mass spectrometry, qualitatively detect the composition of fatty acid and quantitatively detect the content of trace elements of Zn and Mn. The best extraction technology conditions are: the ethanol concentration is 60 percent and the extraction time is 2.5 hours. The ratio of material liquid is one contrasting fifty. The compositions of fatty acid are: palmitic acid, stearic acid, oleic acid, linoleic acid, gamma linolenic acid, arachidonic acid, and eicosapentaenoic acid. The trace elements of Zn and Mn: the trace elements in the crude products are 140.15ug per gram and 67.69ug per gram, the trace elements in the alcohol extract are 48.87ug per gram and 11.58ug per gram. Experiments show that the alcohol process is reasonable, stable and feasible. Alcohol extraction of fatty acid and the trace elements of Zn and Mn have high contents. It can be further developed for efficacy to medicinal liquor products.

KEYWORDS

Hellgrammites, Alcohol extract, Fatty acid, Trace elements

CITE THIS PAPER

Daifang, Z., Ming, P., Yuyun, C., Ling Y., Study on extraction process of alcohol-soluble ingredients from Hellgrammites(Megaloptera: Corydalidae). Environment, Resource and Ecology Journal (2018) 2: 22-30.

REFERENCES

[1] Jepson, J. E., and S. W. Heads. Fossil Megaloptera (Insecta: Neuropterida) from the Lower Cretaceous Crato Formation of Brazil. Zootaxa, 2016, 4098 (1):134-144.
[2] Cao, CQ. Situation of developing and utilizing crawling worm in china and species definition. Hubei Agricultural Sciences,2014, 21: 5061-5064.
[3] Li Yong, Wang Y, Zhao M, et al. Determination of nutrient composition of larvae of Macrobrachium orientalis [J]. Guizhou Agricultural Sciences, 2015, 43 (4): 196-197.
[4] Wang Fubin, Liu Yusheng. Development and utilization value of climbing bug ([J].) agricultural knowledge, 2009, (21): 38-39.
[5] Shi ZX. Survey of the field living environment of the special aquatic reptiles in Panxi [J]. Journal of Xichang University (Natural Science Edition), 2011, 25 (2): 46-50.
[6] Cheng XH. Multifunctional medicinal liquor and its preparation for rheumatic diseases[P]. Chinese patent: CN 101380345A, 2009-03-11.
[7] Yang, D., S. Tao, L. He, C. Wang and J. Li. Effects of protohermes grandis extract on reproductive capacity and life-span of drosophila melangogaste. Nat Prod Res Dev, 2012 24:366-369.
[8] Yang, D., J. Liang, P. Liao, C. Long, L. Luo and J. Li. Effects of protohermes grandis extract on growth and reproduction of mice. Nat Prod Res Dev, 2014, 26:1290-1293.
[9] Li HT, Zhang TS. Analysis of Mixed Fatty Acids by Gas Chromatography-Mass Spectrometry[J]. Leather Chemical Industry, 2003, 20 (6): 18-22.27.
[10] Shu GT, Qu HY, Zou HH,et al. Simultaneous determination of lead and mercury in cosmetics by leaching-nitration[J].Chinese Journal of Health Inspection, 1992, 2(5): 309-310.
[11] LI SM, LI ML. Extraction of various insect oils, analysis of fatty acids and separation and purification of alpha-linolenic acid[J]. Southwest University of Agriculture and Forestry, 2006, 6 (17): 20-21.
[12] Zhao Yuqi. Mass Spectrometric Analysis of Fatty Acid Methyl Ester[J]. National Center for Food Quality and Safety Supervision and Inspection, 2010, 29:132-135.
[13] LIU XY. A systematic taxonomic study of the Chinese panoptera (Insecta: Venipelata) [D]. Beijing: China Agricultural University, 2008.
[14] Value of climbing sand bug in Heuhui River, [EB/OL]. http//nc.mofcom.mofcom.gov.cn/arti-
clep/px/njbk/tzty/tztylzjs/201212/1841259_1.html. 2012-12-05.
[15] Liu LR. A brief introduction to the resources and animals in Liangshan area and some suggestions on their protection and utilization[J]. Exploration of Nature, 1983 (4): 118-123.[8]
[16] Mossoba M M, Mcdonald R E, Roch J A G, et al.. Spectral confirmation of trans monounsaturated C<sub>18</sub> fatty acid positional isomers[J]. Journal of the American Oil Chemists Society, 1997, 74(2): 125-130.
[17] Patrikios I S, Patsalis P C. Monounsaturated fatty acid oligomerization is responsible for the agglutination activity of heated virgin olive oil[J]. Food Research International, 2003, 36(9-10): 985-990.
[18] Ovesen L, Leth T, Hansen K. Fatty acid composition and contents of trans monounsaturated fatty acids in frying fat and in margarines and shortenings maketed in Denmark[J]. Journal of the American Oil Chemists Society, 1998, 75(9): 1079-1083.
[19] Zhu LY, Zhang XC, Song XJ, et al. [J]. Marine Science, 2007, 31 (11): 78-79.
[20] Yu NC, Guan JH. Analysis and Study on Trace Elements and Therapeutic Effect of 8 Kidney-tonifying Herbs [J]. Shizhen Guoyao Research, 1997, 8 (6): 510-511.

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Study on extraction process of alcohol-soluble ingredients from Hellgrammites(Megaloptera: Corydalidae)

Author(s)

Affiliation(s)

Corresponding Author

ABSTRACT

KEYWORDS

CITE THIS PAPER

REFERENCES

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