Plant Species Diversity of Some Wadis at Red Sea Coast, Egypt

The present study aims to investigate the variation in vegetation and species diversity in two coastal wadis which drain their water from surrounding high mountains into the Red Sea. It attempted to compare the floristic diversity between these wadis to recognize the different distribution patterns of species, and to assess the role of the edaphic factors which control the distribution of the plant communities. Fourteen sample plots were selected to represent as much as possible the variation in the vegetation, and georeferenced using GPS techniques. A total of 23 perennial species belonging to 19 genera and 14 families were recorded, with Zygophyllaceae, Fabaceae, Tamaricaceae and Asteraceae represented the species-rich families. Phanerophytes and Chamaephytes constituted the main bulk of the flora, followed by hemicryptophytes. As part of the Saharo-Arabian region, the Saharo-Arabian chorotype dominated the others. Classification of the vegetation resulted in 6 vegetation groups: (A) Juncus rigidus, (B) Zygophyllum coccinium, (C) Capparis decidua, (D) Zilla spinosa, (E) Zygophyllum coccinium and (F) Nitraria retusa. Species richness was positively correlated with fine gravel, while negatively correlated with medium sand. The Shannon’s diversity and Simpson Indices showed positive correlation with CaCO3 and negative correlation with fine sand. Vegetation-soil relationships were assessed by canonical correspondence analysis (CCA) using 18 soil factors indicated that gravel, coarse sand, fine sand, silt, clay, sodium and chlorides were the key soil variables that affect the distribution of plant communities in the coastal wadis of Red Sea region. Received: 10/05/2017 Accepted: 01/06/2017 Published: 04/06/2017


Introduction
The vegetation in Egypt's deserts is by far the most important and characteristic type of natural plant life.It covers about 95% of the total area of the country, and is mainly formed of xerophytic shrubs and sub-shrubs.[1] Monod recognized two types of desert vegetation, namely contracted and diffuse.Both types refer to permanent vegetation that can be accompanied by ephemeral (or annual) plant growth depending on the amount of precipitation in a given year.[2; 3] Kassas added a third type termed "accidental vegetation", where precipitation is very low and falls so irregularly that no permanent vegetation exists.The most critical gradients in abiotic factors may be related to water availability, including annual precipitation, topography and soil properties [4].
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Study
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Vegetation survey
Vegetation survey and assessing the ecological situation of plants were carried out in the summer season of the year 2016 at two studied wadis namely Wadi El-Mallaha and Wadi Esli.fourteen stands (7 stand, each) have been selected for sampling vegetation.These stands were randomly chosen at locations where considerable vegetation cover was encountered.The density and cover of each species have been estimated in each selected stand [16].Relative values of density and cover were calculated for each plant species and summed up to give an estimate of its important value (IV) in each stand which is out of 30.In this extreme arid desert environment, where the vegetation comprised only of widely spaced shrubs and trees, the size of studied sample plots was 10 m x 10 m.The recorded taxa were classified according to the life-form system that proposed by [17; 18].The number of species within each life form category was expressed as a percentage of total number of species in the study area.Analysis of phytogeographical ranges was carried out using [19; 20; 21].Taxonomic nomenclature was according to [22; 23; 24].

Soil analysis
For each sampled stand, three soil samples were collected from profiles of 0-40 cm depth.These samples were then pooled together to form one composite sample, air dried and thoroughly mixed.Textures were determined by sieving method to separate gravels, coarse sand, fine sand, silt and clay for physical and chemical analyses [25].Soil-water extracts (1:5) were prepared for the determination of electric conductivity and pH.Determination of calcium and magnesium were carried out by titration methods with 0.01 N EDTA [26].Sodium and potassium were determined using flame photometer technique [27].Estimation of chlorides was carried out by titration methods using 0.005 N Silver Nitrate [28; 29].

Data analyses
A floristic data matrix of 14 stands and 23 species was subjected for classification by two way indicator species analysis (TWINSPAN, version 2.3) [30].The relation between the vegetation and soil gradients was assessed using the appropriate ordination method to perform direct gradient analysis Canonical Correspondence Analysis (CCA) [31].Plant diversity indices included species richness (SR), Shannon-Weiner diversity index (H´) and Simpson index.These diversity indices were estimated for each vegetation group [32; 33; 34].Taxonomic diversity including species/genera and genera/families ratios for studied area as a whole was determined [35].The obtained data were statistically evaluated using COSTAT 6.4 for Windows.The floristic diversity of study area showed that a total of 23 species belonging to 19 genera and 14 families were recorded (Table 2).The species/genera ratio is 1.21% and genera/families ratio is 1.3%.Generally, The largest family was Zygophyllaceae (4 species), Fabaceae and Tamaricaceae (3 species, each) and Asteraceae (2 species).They constituted 52.2% of the recorded species, and represent most of the floristic structure in the studied wadis.Nine families were represented by only one species.The largest genera were Tamarix (3 species) and Zygophyllum and Acacia (2 species, each).Phanerophytes and Chamaephytes (39.1%, each) constituted the largest number of species (9 species each) and hemicryptophytes (21.7%).Obviously, genus with higher number of species included Tamarix (3 species).Another nine genera were represented by two species, including, Acacia and Zygophyllum.

Floristic analysis
Chorological analysis of the surveyed flora revealed that 11 species (47.8% of the total flora) were monoregional native to Saharo-Arabian chorotype.Thus it forms the major component of the floristic composition of this study (Table 2).About 39.1% of the recorded species were biregional and pluriregional extending their distribution all over the Mediterranean, Saharo-Arabian, Irano-Turanian regions.Being part of Saharo-Arabian region, the Saharo-Arabian chorotype (Mono-; biand pluri-) constitutes 47.8% and 30.4% and 13% of the recorded species, respectively.The two studied wadis shared 5 species or about 21.7% of the total recorded species (23) (Table 3).Among the recorded species Acacia tortilis subsp.raddiana, Achellia fragrentisma, Nitraria retusa, Zilla spinosa and Zygophyllum coccinium.Eighteen species showed a degree of consistency to a certain wadi, and distributed as follows: 12 species to Wadi Esli and 6 species to Wadi El-Mallaha (Table 3).Three ubiquitous (have a wide ecological range of distribution) were recorded: Zygophyllum coccinium, Zilla spinosa and Acacia tortilis subsp.raddiana have the highest presence values (P = 100%, 71.4% and 35.7%, respectively).On the other hand, the annuals are absent, demonstrated a certain degree of constancy.

Classification of vegetation
Application of classification using cluster analysis to the floristic data of the studied wadis yielded 6 vegetation groups (Figure 2).Zygophyllum coccinium is recorded with variable presence values in all the 6 groups (Table 2).Significant differences in the examined soil variables within the separated vegetation groups were demonstrated in (Table 4), such as Moisture, Electric conductivity and chlorides showed clear significant differences between groups at p < 0.05.

Group (A): Juncus rigidus group
The 8 species in this group were recorded in two stands with average species richness of 4.00±0.00species per sample plot, Shannon-Wiener diversity index of 1.29±0.14and Simpson index of 0.41±0.04(Table 4).Stands of this group were found on soil rich in its electric conductivity, fine sand, moisture contents, Na, Cl and Mg. the abundant species was Juncus rigidus (IV= 140.4) and the associated species were Phoenix dactylifera, Alhagi graecourum, Nitraria retusa, Aeluropus lagopoides, Phragmites australis, Tamarix nilotica and Zygophyllum coccinium (Table 5).

Group (B): Zygophyllum coccineum group
The size of this group was represented by 2 stands that included 4 species.The average species richness in this group is 2.50±0.71species/stands, Simpson index 0.22±0.02and Shannon-Wiener diversity index of 0.67±0.070.The stands of this group inhabited soil with the highest content of potassium, coarse and medium sand (Table 4).Sporadic species included 4 species, of which Zilla spinosa, Tamarix nilotica, Balanites aegyptiaca and Zygophyllum coccinium were included (Table 5).The abundant species include Zygophyllum coccinium (IV = 115.9).

Group (C): Capparis decidua group
The 5 species in this group were recorded from 3 stands, with average species richness of 3.33±0.58species per sample plot, Shannon diversity index of 1.07±0.2and Simpson index 0.34±0.07.The stands of this group were found on soil with high content of gravel and fine gravel, and lowest contents of Silt, sodium, potassium, chlorides and calcium carbonates (Table 5).Five sporadic species were recorded that included amongst others, Pulicaria undulata, Zilla spinosa, Capparis decidua, Leptadenia pyrotechnica and Zygophyllum coccinium (Table 2).The abundant species was Capparis decidua (IV= 107.9).

Group (D): Artemisia judaica group
This group comprised 7 species recorded from 1 stand, it is the most diversified group with an average species richness of 5.00 species per sample plot, Shannon-wiener diversity index of 1.80 and Simpson index 0.57 (Table 5).The stands of this group characterized by soil with the highest coarse sand and calcium, the lowest contents of fine sand, moisture content, pH, electric conductivity, chlorides.The low number of studied stands in this group was due to rugged topography and limited accessibility.(Table 2).The abundant species was Zilla spinosa (IV= 72.1). the associated species were Aerva javanica, Achellia fragrentisma, Cleome dreserifolia, Forsskaolea tenacissima, Fagonia arabica and Zygophyllum coccinium.

Ordination of sampling plots
Application of Principal Component Analysis (PCA) resulted in the segregation of the 6 vegetation groups that were obtained from classification, with most of their sampling plots within, along the first two axes (eigenvalues 0.14 and 0.17 for axis 1 and 2, respectively) (Figure 3).Plots of group (A) were separated along the positive ends of the PCA axis 1, while those of groups (D) and (E) were separated along its negative end.On the other hand, plots of groups (B) and (F) were separated along the positive ends of the PCA axis 2, while those of groups (C) was separated along its negative end.

Soil-vegetation relationships
The relationship between the vegetation and soil variables was studied using Canonical Correspondence Analysis (CCA).Figure (4) showed the CCA ordination biplot with vegetation.Groups (A-F), and the examined soil variables.It can be noted that sampling plots of groups (A) was highly correlated with conductivity, fine sand, moisture contents, Na, Cl and Mg.On the other hand, plots of group (B) was highly associated with potassium, coarse and medium sand.Group (C) was related to gravel and fine gravel.Group D was highly associated with coarse sand and calcium.Group E was highly correlated with sulphate and Organic matter.Group F was related to silt, clay and CaCO 3 .

Discussion
The desert vegetation in arid zones is relatively homogeneous but in small spatial scale is heterogeneous.Therefore, the studies of vegetation-soil relationships in these areas were usually conducted in small spatial scale [36; 37; 38].The vegetation in the study area is restricted to wadis, runnels and/or depressions where runoff water collects and provides sufficient moisture for plant growth [1; 19; 39].[2] Kassas found that the vegetation of the Red Sea wadis is characterized by sparseness of plant cover, there are limited number of plant species (mean species richness 4.5 ± 1.95 on the average in studied sampling plots), The vegetation structure in the studied area is relatively simple, in which the species have to withstand the harsh environmental conditions.This is reflected to the absence of annuals and presence of several highly adapted, drought-resistant species [40].
The life form spectra provide information which may help in assessing the response of vegetation to variations in environmental factors [41].The present study demonstrated that phanerophytes and chamaephytes constituted the largest number of species with value (39.1%, each), that agreed with those of other reports [42; 43; 44; 45].The highest values of phanerophytes and chamaephytes may be attributed to the ability of species to resist drought, salinity, sand accumulation and over grazing, also the absence of therophytes is response to the harsh climate, topography variation and biotic influence [46].
Chorological analysis of the floristic data revealed that the Saharo-Arabian chorotype forms the major component of the floristic structure where it was represented by 47.8%.This is in accordance with the results obtained in [43; 47].This may be attributed to the fact that plants of the Saharo-Arabian species are good indicators for desert environmental conditions.Spatial distribution of plant species and communities over a small geographic area in desert ecosystems is mainly related to topography and landform pattern [48].In this context, the variations in local topography, edaphic factors and microclimatic conditions lead to the diversity of the plant communities in the study area.Classification of the vegetation resulted in 6 vegetation groups: (A) Juncus rigidus, (B) Zygophyllum coccinium, (C) Capparis decidua, (D) Zilla spinosa,(E) Zygophyllum coccinium and (F) Nitraria retusa.Most of the identified vegetation groups have similar vegetation units with that recorded in some wadis of the Eastern Desert [5; 42; 43; 49], Western Desert [50; 51] and western Mediterranean region [52; 53], in south Sinai region [54; 55].
Several studies have provided qualitative assessments of the distribution of plant species and associations in relation to physiographic factors in different areas of the Eastern desert, In this study, soil-vegetation relationships the inland wadis revealed that gravel, coarse sand, fine sand, silt, clay, sodium and EC were the most important soil factors.This is in accordance with other relevant works in wadis of the arid and semi-arid regions.Soil texture, salinity and organic carbon can affect phytodiversity of wild communities [56; 57].These findings are also in agreement with the results obtained from this study.

Figure 2 Figure 3 :
Figure 2 temperature of the hottest month 20-30°C).The mean annual rainfall ranges from 25 mm in Suez, 4 mm in Hurghada to 3.4 mm in El-Qusseir and 1.2 mm in Mersa Alam.On daily basis, the mean minimum temperature is high and ranges between 18 and 22.3°C in and mean maximum 27.8-29.5°C.Relative humidity ranges from 46.1% in Hurghada to 54.4% in Mersa Alam.The wind velocity ranges between 9.55 in Mersa Alam and 11.7 in El-Quseir.Some climatic features in certain stations along the Red Sea coast and the Nile Valley may explain environmental aridity and thermal continentality which increases from north to south (Table1

Table 1 :
). Annual average (over 50 years) of some climatic features at Four meteorological stations within the study area.(Max=maximum, Min= Minimum).according to Climatic Normal of Egypt.

Table 3 :
Comparison between the floristic compositions in the 2 studied wadis (Figures are the presence percentages of species).

Table 4 :
Mean values, standard deviations (±) and ANOVA values of the soil variables in the vegetation groups (A-E) of the inland wadis.EC = Electric conductivity, TSS = Total soluble salts, G = Gravel, FG= Fine gravel, CS = Coarse sand, MS= Medium sand, FS = Fine sand, SR = Species richness, H = Shannon-Wiener index and S= Simpson index.

Table 5 :
Floristic composition in the vegetation groups in the study area.Values in bold are species with highest presence values.

Table 6 :
The results of ordination for the four CCA axes, intraset correlation of the soil variables, together with eigenvalues and species-environment correlation in the two studied wadis.For abbreviations and units, see Table4.

Table ( 7
): Correlation coefficients between soil factors and diversity indices of two studied wadis.