Author(s)

Li Rui ¹

Affiliation(s)

¹ Focused Photonics (Hangzhou) Inc, Hangzhou, Zhejiang, China

Corresponding Author

Li Rui

ABSTRACT

Spectral flow cytometry is an important biomedical instrument to be used for the quantification and analysis of cell surface markers. This paper studies the data acquisition and processing technology of spectral flow cytometry to improve the data quality and analysis effect. First, the principle and application fields of spectroscopic flow cytometry are presented. Then, key issues in the data acquisition process, including laser power regulation, instrument calibration, and sample preparation, are discussed. Then, common data processing methods, including peak identification, data cleaning and normalization. Finally, the effectiveness and feasibility of the proposed data acquisition and processing techniques are verified by experiments. This study provides useful guidance for the accurate acquisition and reliable analysis of spectral flow cytometric data.

KEYWORDS

Spectral flow cytometry, data acquisition, data processing, peak identification, data cleaning

CITE THIS PAPER

Li Rui, Research on the data acquisition and processing technology of spectral flow cytometry. MEDS Clinical Medicine (2024) Vol. 5: 20-25. DOI: http://dx.doi.org/10.23977/medsc.2024.050104.

REFERENCES

[1] Tan Guozhen. Research on key technologies of single laser multiccolor flow cytometry [D]. Changchun University of Science and Technology, 2023.
[2] Li Xing. Spectral flow cytometry data acquisition and processing technology study [D]. Xiamen University, 2021.
[3] Fang Xiaoling. Development of a spectral flow cytometry fluorescence acquisition system [D]. Xiamen University, 2018.

Sponsors, Associates, and Links

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