Author(s)

Yuanyuan Su ¹, Jingjing Li ¹, Limin Chen ¹, Yanan Wang ², Hongnan Lian ³, Yamin Qie ⁴

Affiliation(s)

¹ Department of Obstetrics and Gynecology, Affiliated Hospital of Hebei University, Baoding, Hebei, 071000, China
² Department of Obstetrics and Gynecology, Rongcheng County People's Hospital, Baoding, Hebei, 071700, China
³ Department of Obstetrics and Gynecology, Baoding Maternal and Child Health Hospital, Baoding, Hebei, 071000, China
⁴ Department of Obstetrics and Gynecology, Wangdu County Hospital of Traditional Chinese Medicine, Baoding, Hebei, 071000, China

Corresponding Author

Yuanyuan Su

ABSTRACT

Gestational diabetes mellitus (GDM) is a serious pregnancy complication that impacts both the mother's and the infant's health. Its incidence is escalating annually, posing a risk to the well-being of expectant mothers and newborns. This paper delves into the methylation patterns of the Pancreatic and Duodenal Homeobox 1 (PDX1) gene, exploring its link to pregnancy outcomes in GDM patients. Methodologically, we rigorously selected participants based on specific inclusion and exclusion criteria, gathering detailed clinical and biochemical data. We employed methylation-specific PCR and real-time fluorescence quantitative PCR to assess the methylation status of the PDX1 gene, as well as the expression levels of Neurogenin 3 (NGN3) and Pax6. Additionally, we meticulously evaluated pregnancy outcomes. The results indicate that GDM patients exhibit a notably higher methylation level of the PDX1 gene compared to healthy pregnant women. This increased methylation correlates negatively with poorer pregnancy outcomes. Furthermore, our study revealed substantial changes in NGN3 and Pax6 expression levels in GDM patients, which are closely tied to pregnancy outcomes. In summary, our findings offer fresh insights into the pathogenesis of GDM and suggest potential targets for improving pregnancy outcomes.

KEYWORDS

Gestational Diabetes Mellitus; Methylation of PDX1 gene; Pregnancy outcome

CITE THIS PAPER

Yuanyuan Su, Jingjing Li, Limin Chen, Yanan Wang, Hongnan Lian, Yamin Qie, Effect of Methylation Status of PDX1 Gene on Pregnancy Outcome in Patients with Gestational Diabetes Mellitus. MEDS Clinical Medicine (2024) Vol. 5: 120-125. DOI: http://dx.doi.org/10.23977/medsc.2024.050219.

REFERENCES

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