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  4. Vol 6, Issue 3, 2024

Study on the Pharmacological Material Basis and Mechanism of Action of Yangwei Qingre Huayu Decoction in the Treatment of Chronic Atrophic Gastritis Based on LC-MS and Network Pharmacology

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DOI: 10.23977/medcm.2024.060318 | Downloads: 13 | Views: 826

Author(s)

Mengmeng Ye 1, Xinli Wen 1, Jingyi Bai 2

Affiliation(s)

1 Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, 712046, China
2 Shaanxi Provincial Hospital of Chinese Medicine, Xi'an, Shaanxi, 710003, China

Corresponding Author

Xinli Wen

ABSTRACT

In order to investigate the bioactive substances and potential mechanisms of action of Yangwei Qingre Huayu Decoction (YQHD) in the treatment of chronic atrophic gastritis (CAG) using ultra-high-performance liquid chromatography coupled with quadrupole high-resolution mass spectrometry (UHPLC-Q-Exactive-MS/MS) combined with network pharmacology methods. UHPLC-Q-Exactive-MS/MS was used to obtain total ion chromatograms of the YQHD aqueous extract under different ion modes. The chemical constituents of the extract were rapidly identified, and potential active components were determined through literature review and database searches. Based on network pharmacology, a network of key components and critical targets was constructed. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to identify key signaling pathways and targets. Resultly, a total of 162 active components were identified in YQHD, including salvianolic acids, ursolic acid, and tetrahydropalmatine. GO and KEGG analyses revealed core targets such as tumor protein P53 (TP53), heat shock protein HSP90AA1, and signal transducer and activator of transcription 3 (STAT3). Key pathways included the TNF signaling pathway and proteoglycans in cancer. In conclusion, the results suggest that YQHD may exert therapeutic effects on CAG through multi-component, multi-target, and multi-pathway mechanisms, providing scientific support for its further clinical application. 

KEYWORDS

Yangwei Qingre Huayu Decoction (YQHD); Chronic Atrophic Gastritis (CAG); UHPLC-Q Exactive Focus MS/MS; Network Pharmacology

CITE THIS PAPER

Mengmeng Ye, Xinli Wen, Jingyi Bai, Study on the Pharmacological Material Basis and Mechanism of Action of Yangwei Qingre Huayu Decoction in the Treatment of Chronic Atrophic Gastritis Based on LC-MS and Network Pharmacology. MEDS Chinese Medicine (2024) Vol. 6: 124-135. DOI: http://dx.doi.org/10.23977/medcm.2024.060318.

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