Author(s)

Zizheng Wu ¹, Xing Chen ¹, Jiahao Ye ¹, Xiaoyi Wang ¹, Zhixi Hu ¹

Affiliation(s)

¹ College of Chinese Medicine, Hunan University of Chinese Medicine, No. 300, Bachelor Road, Yuelu District, Changsha, Hunan, 410208, China

Corresponding Author

Zhixi Hu

ABSTRACT

This study aimed to investigate the protective effects and mechanism of Xiangdan injection (XDI) in treating acute myocardial ischemia (AMI) induced by isoproterenol (ISO). To this end, this study employed UPLC-Q-TOF-MS technology, network pharmacology, molecular docking technology, and animal experiment verification.Based on the review of the research literature, XDI absorption into blood components was screened and verified using the UPLC-Q-TOF-MS technology. The software Cytoscape 3.7.2 and the Applied Protein-Protein Interaction Network Analysis Database (STRING) were utilized to build a protein-protein interaction (PPI) network. The software examines the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway to identify common targets. Autodock vina software was used to verify the molecular docking between the main active ingredients and key targets. A rat model of AMI was developed through the intraperitoneal injection of XDI at low and high doses (2.7 and 5.4 ml/kg) for 7 consecutive days, 85 mg/kg of isoproterenol was injected subcutaneously into the back of the rats On the 6^th day.The UPLC-Q-TOF-MS analysis revealed that XDI absorbed 9 blood components. The results also showed that 128 common targets were found in the "XDI-AMI" combination. KEGG enrichment analysis demonstrated that the treatment of AMI by XDI may be related to disease pathways such as lipid and atherosclerosis and TNF signaling pathway. The study findings suggested that XDI can improve the function of cardiomyocytes and, thereby, treat AMI by regulating oxidative stress and inflammatory responses.

KEYWORDS

XiangDan injection; Acute myocardial ischemia (AMI); Network pharmacology; Molecular docking; Inflammatory response; UPLC-Q-TOF-MS

CITE THIS PAPER

Zizheng Wu, Xing Chen, Jiahao Ye, Xiaoyi Wang, Zhixi Hu, An Investigation into Mechanism of XiangDan Injection for the Improvement of Inflammation and Oxidative Stress in Patients with Acute Myocardial Ischemia. MEDS Chinese Medicine (2024) Vol. 6: 91-105. DOI: http://dx.doi.org/10.23977/medcm.2024.060413.

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