Author(s)

Lei Zhang ¹, Feng Yang ², Junzhe Zhu ³

Affiliation(s)

¹ Zhejiang Gongshang University, Hangzhou, Zhejiang, 310018, China
² Community Health Service Center, Hangzhou, Zhejiang, 310000, China
³ Wenzhou Medical University, Wenzhou, Zhejiang, 310000, China

Corresponding Author

Lei Zhang

ABSTRACT

The H. pylori Antigen Rapid Test Cassette (Feces) is a chromatographic immunoassay developed for the qualitative detection of Helicobacter pylori (H. pylori) antigens in human fecal specimens. This research intends to conduct a systematic assessment of its performance metrics, such as sensitivity, specificity, accuracy and clinical applicability, in comparison with another commercial rapid test. A total of 180 fecal samples were tested, consisting of 85 H. pylori antigen-positive and 95 H. pylori antigen-negative specimens. The outcomes revealed high relative sensitivity 97.6%, 95%, specificity 97.9% and overall accuracy 97.8%. The test exhibited no cross-reactivity with various common bacteria and viruses, along with excellent intra-assay and inter-assay precision, achieving over 99% correct identification of samples. These results suggest that the H. pylori Antigen Rapid Test Cassette (Feces) is a reliable, rapid, and practical tool for detecting H. pylori antigens, which can facilitate the timely diagnosis and management of H. pylori-related gastrointestinal diseases.

KEYWORDS

Helicobacter Pylori; Antigen Rapid Test; Fecal Specimen; Chromatographic Immunoassay; Gastrointestinal Diseases

CITE THIS PAPER

Lei Zhang, Feng Yang, Junzhe Zhu, User-Friendly Self-Testing Evaluation of H. Pylori Antigen Detection in Feces. MEDS Clinical Medicine (2025) Vol. 6: 104-108. DOI: http://dx.doi.org/10.23977/medsc.2025.060317.

REFERENCES

[1] Marshall, BJ, McGechie, DB, Rogers, PAR and Glancy, RG. Pyloric Campylobacter infection and gastroduodenal disease. Med. J. Australia. (1985), 149: 439-444.
[2] Soll, AH. Pathogenesis of peptic ulcer and implications for therapy. New England J. Med.(1990), 322: 909-916.
[3] Hazell, SL, et al. Campylobacter pylori is and gastritis I: Detection of urease as a marker of bacterial colonization and gastritis. Amer. J. Gastroenterology. (1987), 82(4): 292-296.
[4] Cutler AF. Testing for Helicobacter pylori in clinical practice. Am j. Med. 1996; 100:35S-41S.

Sponsors, Associates, and Links

---

• Journal of Neurobiology and Genetics
  
  
• Medical Imaging and Nuclear Medicine
  
  
• Bacterial Genetics and Ecology
  
  
• Transactions on Cancer
  
  
• Journal of Biophysics and Ecology
  
  
• Journal of Animal Science and Veterinary
  
  
• Academic Journal of Biochemistry and Molecular Biology
  
  
• Transactions on Cell and Developmental Biology
  
  
• Rehabilitation Engineering & Assistive Technology
  
  
• Orthopaedics and Sports Medicine
  
  
• Hematology and Stem Cell
  
  
• Journal of Intelligent Informatics and Biomedical Engineering
  
  
• MEDS Basic Medicine
  
  
• MEDS Stomatology
  
  
• MEDS Public Health and Preventive Medicine
  
  
• MEDS Chinese Medicine
  
  
• Journal of Enzyme Engineering
  
  
• Advances in Industrial Pharmacy and Pharmaceutical Sciences
  
  
• Bacteriology and Microbiology
  
  
• Advances in Physiology and Pathophysiology
  
  
• Journal of Vision and Ophthalmology
  
  
• Frontiers of Obstetrics and Gynecology
  
  
• Digestive Disease and Diabetes
  
  
• Advances in Immunology and Vaccines
  
  
• Nanomedicine and Drug Delivery
  
  
• Cardiology and Vascular System
  
  
• Pediatrics and Child Health
  
  
• Journal of Reproductive Medicine and Contraception
  
  
• Journal of Respiratory and Lung Disease
  
  
• Journal of Bioinformatics and Biomedicine