Optimization for the therapeutic application of CRISPR/Cas 19 technologies for CHB
DOI: 10.23977/phpm.2022.020101 | Downloads: 36 | Views: 1348
Jasmine Wang 1
1 St. Andrew's Episcopal School, Potomac, MD, 20854
Corresponding AuthorJasmine Wang
Hepatitis-B Virus (HBV) is able to escape the immune system by converting HBV DNA into covalently closed circular DNA (cccDNA). This allows an acute Hepatitis B infection to evolve into a chronic Hepatitis B infection which may lead to life-long illness. After the discovery of the CRISPR/Cas9 system, different trials have been done to eliminate the cccDNA but none of them have been very effective. Based on a previous study, I propose a new methodology by which the CRISPR administration by replacing dual Adeno-Associate virus (AAV) vector and add another single-guide RNA (sgRNA) and accorded promoter to intensify the affect.
KEYWORDSHepatitis B, CRISPR/Cas9, covalently closed circular DNA, adeno-associated virus, sgRNA, PAMs
CITE THIS PAPER
Jasmine Wang, Optimization for the therapeutic application of CRISPR/Cas 19 technologies for CHB. MEDS Public Health and Preventive Medicine (2022) Vol. 2: 1-5. DOI: http://dx.doi.org/10.23977/phpm.2022.020101.
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