Study on the molecular mechanism of Huang jing in the treatment of type 2 diabetes mellitus based on network pharmacology
DOI: 10.23977/medcm.2021.030101 | Downloads: 3 | Views: 221
Hui Zhang 1, Taofeng Lu 1, Yanjun Wu 1, Jing Zhang 1, Shuguang Wu 1
1 Guizhou University of Traditional Chinese Medicine, Guizhou Guiyang, 550002
Corresponding AuthorShuguang Wu
Objective To explore the possible mechanism of Huang jing in the treatment of T2DM by using network pharmacology. Methods Using TCMSP database to obtain the active components and targets of Xanthine, combined with OMIM, GeneCards, DrugBank, DisGeNET and other databases to screen T2DM-related gene targets, extracting drug-disease target intersection with R software, and constructing drug-component-disease-target network diagram. The intersection target PPI network is constructed through the String database and Cytoscape software. The GO function and KEGG pathway enrichment analysis of the target of T2DM are performed using R software and Metascape database. Results 12 active ingredients and 76 targets are selected from Huang jing. Key proteins involved AKT1, VEGFA, PPARG, CASP3, FOS, MAPK14, MMP9, etc. 818 items are obtained by GO functional enrichment analysis, KEGG pathway enrichment screening obtained 80 signaling pathways is mainly concerned with the signaling pathways including whereas resistance, age-rage signaling pathway in the diabetic outcome, MAPK signaling pathway, and Relaxin signaling pathway. Conclusion The possible mechanism of action of xanthine in the treatment of T2DM is predicted by using network pharmacology method. The useful components of Xanthine mainly coordinated the intervention of T2DM by coordinating inflammatory signaling pathway, insulin-related pathway and antioxidation-related signaling pathway, providing new ideas and clues for further clinical application research.
KEYWORDSHuang jing, Type 2 diabetes, Network pharmacology, Active ingredients, Molecular mechanisms
CITE THIS PAPER
Hui Zhang, Taofeng Lu, Yanjun Wu, Jing Zhang, Shuguang Wu, Study on the molecular mechanism of Huang jing in the treatment of type 2 diabetes mellitus based on network pharmacology. MEDS Chinese Medicine (2021) Vol. 3: 1-10. DOI: http://dx.doi.org/10.23977/medcm.2021.030101
 Dai Qinghong, Wang Zhongdong. The status of diabetes mellitus in China [J]. Chinese Journal of Medicine, 2011, 9 (13): 206-208.
 Xu Qinghai. Study on the therapeutic effect of type 2 diabetes mellitus [J]. Medical Equipment, 2017, 30 (04): 191-192.
 Zhou Zhongying, Xue Boyu.Zhou zhongying practical Chinese medicine internal medicine [M]. Beijing: China Press of Traditional Chinese Medicine, 2012: 763-774.
 Zhang Hongmei. Study on the etiology, pathogenesis and treatment of diabetes mellitus (thirst elimination) [J]. World Latest Medical Information Abstracts, 2018, 18 (87): 155+161.
 Tao Aien, Zhang Xiaocan, Du Zefei, et al. Research progress on flavonoids and their pharmacological activities in the genus Chrysosperma [J]. Chinese Herbal Medicine, 2018, 49 (09): 2163-2171.
 XIE W, DU L. Diabetes is an inflammatory disease: evidence from traditional Chinese medicines [J]. Diabetes Obes Metab, 2011, 13 (4): 289-301.
 Xu Jinlong, Chen Wu, Duan Baozhong. Huang Jing Ⅱ diabetes treatment of TCM and Meta analysis [J]. Chinese Journal of Traditional Chinese Medicine, 2017, 35 (07): 1698-1701.
 Fu Tingting, Wang Guoxian, Chen Tingting, et al. Inhibitory effects of polysaccharide from Polygonatum sinensis on renal function of diabetic nephropathy in rats [J]. Pharmacology and Clinics of Traditional Chinese Medicine, 2015, 31 (04): 123-126.
 Wang Xing, Li Caina, Shen Zhufang. Study on the protective effect of baicalein on diabetic peripheral neuropathy [J]. Chinese Journal of Clinical Pharmacology, 2018, 34 (01): 70-72+76.
 Wei Xuefang, Lin Shaobin, Xiong Hongping, et al. Effects of baicalein on pancreatic islet function in diabetic rats [J]. Journal of Cardiovascular and Cerebrovascular Diseases of Integrated Traditional and Western Medicine, 2019, 17 (19): 2933-2935.
 WANG S, YE K, SHU T, et al. Enhancement of Galloylation Efficacy of Stigmasterol and beta-Sitosterol Followed by Evaluation of Cholesterol-Reducing Activity [J]. Journal of Agricultural and Food Chemistry, 2019, 67 (11): 3179-3187.
 PONNULAKSHMI R, SHYAMALADEVI B, VIJAYALAKSHMI P, et al. In silico and in vivo analysis to identify the antidiabetic activity of beta sitosterol in adipose tissue of high fat diet and sucrose induced type-2 diabetic experimental rats [J]. Toxicol Mechanisms and Methods, 2019, 29 (4): 276-290.
 KHOSRAVIA Z, SEDAGHATB R, BALUCHNEJADMOJARADC T, et al. Diosgenin ameliorates testicular damage in streptozotocin-diabetic rats through attenuation of apoptosis, oxidative stress and inflammation [J]. International Immunopharmacol, 2019, 10:37.
 Wu Leitao. Diosgenin regulates ESR and ERK/Akt signaling pathway to promote SCS proliferation [D]. Shaanxi: Doctoral Dissertation of the Fourth Military Medical University, 2015.
 Apoptosis and Apoptosis in human diabetes mellitus [J]. PLoS One, 2014, 6 (4): 453 -- 453.
 Yu Hong, Shi Mingjun, Xiao Ying, et al. Danqi Mixture down-regulates the expression of AKT1 in renal tissue of diabetic nephropathy rats [J]. Chinese Journal of Experimental Formulae, 2013, 19 (07): 259-262.
 RAJ R, BHATTI JS, BHADADA SK, et al. Association of polymorphisms of peroxisome proliferator activated receptors in early and late onset of type 2 diabetes mellitus [J]. Diabetology and Metabolic Syndrome, 2017, 11: S287-S293.
 WANG Lu, ZHANG Ruihu, WANG Chenyang, et al. [J]. Int J Diabetes Res, 2000, 14 (2): 173-183.Chinese Journal of Experimental Zoology, 2019, 27 (04): 508-515.
 Liu Yanmin, Mei Yan, Li Jie, et al. Effects of emotional stimulation on Fos protein in the paraventricular nucleus of hypothalamus in diabetic rats [J]. World Journal of Integrated Traditional Chinese and Western Medicine, 2019, 14 (10): 1396-1399.
 DJORDJEVIC B, CVETKOVIC T, STOIMENOV TJ, et al. Oral supplementation with melatonin reduces oxidative damage and concentrations of inducible nitric oxide synthase, VEGF and matrix metalloproteinase 9 in the retina of rats with streptozotocin/nicotinamide induced pre - diabetes [J]. European Journal of Pharmacology, 2018, 833: 290-297.
 Huang Li, Feng Xuezhen, Xu Bo, et al. Effects of acute myocardial infarction on neuroendocrine-related hormones in patients with type 2 diabetes mellitus [J]. Trauma and Acute Care Medicine, 2018, 6 (06): 412-413+415.
 Bao Weijie, Zhang Wenjian, Xu Shiqing, et al. The role of AGE-RAGE system in diabetic neuropathy [J]. Advances in Physiology, 2014, 45 (02): 137-139.
 CUI X, QIAN DW, JIANG S, et al. Scutellariae Radix and Coptidis Rhizoma Improve Glucose and Lipid Metabolism in T2DM Rats via Regulation of the Metabolic Profiling and MAPK/PI3K/Akt Signaling Pathway [J]. International Journal of Molecular Sciences, 2018, 19 (11): 3634.
 Zong Yang, Dong Hongli, Chen Ting, et al. Effects of Scutellaria baicalensis and Coptis chinensis on the treatment of type 2 diabetes mellitus [J]. Chinese Traditional and Herbal Medicine, 2019, 50 (04): 888-894.