Education, Science, Technology, Innovation and Life
Open Access
Sign In
  1. Home
  2. Journals
  3. MEDS Public Health and Preventive Medicine
  4. Vol 1, Issue 1, 2021

Isolation and Identification of Aquamicrobium Strains from a Fecal Contaminated Sludge Sample

Download as PDF

DOI: 10.23977/phpm.2021.010103 | Downloads: 22 | Views: 2445

Author(s)

Chengru Wu 1, Chunyan le 1, Tianwen Gao 1, Yingsong Zheng 2, Jinjun Ji 3

Affiliation(s)

1 College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
2 The First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
3 College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China

Corresponding Author

Jinjun Ji

ABSTRACT

The metabolism of cholesterol by bacteria may impact human health directly and indirectly. To elucidating the degradation process of cholesterol is of great benefit to understand the relationship between environmental microbes and human health. Isolation and identification cholesterol-degrading microorganisms is a basic work to obtaining superior experimental materials. Serial dilution agar plating and agar plate streaking were used to obtain single and pure bacterial colony. Sanger sequencing, Gram staining, and some physiological and biochemical reaction assays were used to characterize isolated bacteria. Two strains, Aqu1 and Aqu2, were isolated and purified from a fecal contaminated sludge sample. The colonies of Aqu1 and Aqu2 were variety of milky white-color. Both strains were gram negative, spherical shaped Aquamicrobium genus bacteria. The 16s rDNA of Aqu1 and Aqu2 was highly identity to that of A. aestuarii and A. lusatiense, respectively.

KEYWORDS

Cholesterol degradation, Bacterial isolation, Biochemical classification, Physiological classification, 16s rdna taxonomic study

CITE THIS PAPER

Chengru Wu, Chunyan le, Tianwen Gao, Yingsong Zheng, Jinjun Ji, Isolation and Identification of Aquamicrobium Strains from a Fecal Contaminated Sludge Sample. MEDS Public Health and Preventive Medicine (2021) 1: 18-22. DOI: http://dx.doi.org/10.23977/phpm.2021.010103

REFERENCES

[1] Bhatti H.N., Khera R.A. “Biological transformations of steroidal compounds: a review”. Steroids, vol.77, no.12, pp.1267-90, 2012.
[2] Slaytor M., Bloch K. “Metabolic transformation of cholestenediols”. Journal of Biological Chemistry. vol. 240, no. 12, pp.4598-602, 1965.
[3] Miller W.L. “Molecular biology of steroid hormone synthesis”. Endocrine Reviews. vol. 9, no. 3, pp. 295-318, 1988
[4] Björkhem I., Eggertsen G. “Genes involved in initial steps of bile acid synthesis”. Current Opinion in Lipidology. vol. 12, no. 2, pp.97-103, 2001.
[5] Wei S.T., Wu Y.W., Lee T.H., et al. “Microbial Functional Responses to Cholesterol Catabolism in Denitrifying Sludge”. mSystems. vol. 3, no. 5, pp.e00113-18, 2018.
[6] Hudson S.B., Kluever B.M., Webb A.C., et al. “Steroid hormones, energetic state, and immunocompetence vary across reproductive contexts in a parthenogenetic lizard”. General and Comparative Endocrinology. vol. 288, pp.113372, 2020. [online] Available: https://doi.org/10.1016/j.ygcen.2019.113372.
[7] Hoffman M., Mann L.K., Won J.H., et al. “Steroid Hormone Levels in Recipient Amniotic Fluid in Twin-Twin Transfusion Syndrome and Their Association with Preterm Delivery”. American Journal of Perinatology. vol. 37, no. 6, pp.562-569, 2020.
[8] Houshdaran S., Oke A.B., Fung J.C., et al. “Steroid hormones regulate genome-wide epigenetic programming and gene transcription in human endometrial cells with marked aberrancies in endometriosis”. PLoS Genetics. vol. 16, no. 6, pp.1-40, 2020. [online] Available: https://doi.org/10.1371/journal.pgen.1008601
[9] Veiga P., Juste C., Lepercq P., et al. “Correlation between faecal microbial community structure and cholesterol-to-coprostanol conversion in the human gut”. FEMS Microbiology Letters. vol. 242, no. 1, pp.81-86. 2005.
[10] Oren A., Garrity G.M. “Then and now: a systematic review of the systematics of prokaryotes in the last 80 years”. Antonie Van Leeuwenhoek. vol. 106, no. 1, pp.43-56, 2014.
[11] Bambauer A., Rainey F.A., Stackebrandt E., et al. “Characterization of Aquamicrobium defluvii gen. nov. sp. nov., a thiophene-2-carboxylate-metabolizing bacterium from activated sludge”. Archive of Microbiology. vol. 169, no. 4, pp. 293-302. 1998.
[12] Kämpfer P., Martin E., Lodders N., et al. “Transfer of Defluvibacter lusatiensis to the genus Aquamicrobium as Aquamicrobium lusatiense comb. nov. and description of Aquamicrobium aerolatum sp. nov.”. International Journal of Systematic and Evolutionary Microbiology. vol. 59, Pt10, pp.2468-2470. 2009.
[13] Jin H.M., Kim J.M., Jeon C.O. ”Aquamicrobium aestuarii sp. nov., a marine bacterium isolated from a tidal flat”. International Journal of Systematic and Evolutionary Microbiology.  vol. 63, pp.4012-4017, 2013.
[14] Lipski A., Kampfer P. ”Aquamicrobium ahrensii sp. nov. and Aquamicrobium segne sp. nov., isolated from experimental biofilters”. International Journal of Systematic and Evolutionary Microbiology.  vol. 62, pp.2511-2516, 2012.
[15] Fritsche K., Auling G., Andreesen J.R., et al. “Defluvibacter lusatiae gen. nov., sp. nov., a new chlorohenol-degrading member of the alpha-2 subgroup of proteobacteria”. Systematic and Applied Microbiology. vol. 22, pp.197-204, 1999.
[16] Xu C.F., Zhang L., Huang J.W., et al. “Aquamicrobium soli sp. nov., a bacterium isolated from a chlorobenzoate-contaminated soil”. Antonie Van Leeuwenhoek. vol. 110, pp.305-312, 2017.
[17] Wu Z.G., Wang F., Gu C.G., et al. “Aquamicrobium terrae sp. nov., isolated from the polluted soil near a chemical factory”. Antonie Van Leeuwenhoek. vol. 105, no. 6, pp.1131-1137.2014.
[18] Wang X., Jin D., Zhou L., et al. “Draft Genome Sequence of Aquamicrobium defluvii Strain W13Z1, a Psychrotolerant Halotolerant Hydrocarbon-Degrading Bacterium”. Genome Announcements. vol. 3, no. 4, pp.e00984-15. 2015. [online] Available: https://doi: 10.1128/genomeA.00984-15.
[19] Chang Y.C., Sawada K., Kim E.S., et al. “Whole-Genome Sequence of Aquamicrobium sp. Strain SK-2, a Polychlorinated Biphenyl-Utilizing Bacterium Isolated from Sewage Sludge”. Genome Announcements. vol. 3, no. 3, pp.e00439-15. 2015. [online] Available: https://doi: 10.1128/genomeA.00439-15.
[20] Sugawara H., Koyama D., Sawada K., et al. “Purification and degradation characteristics of biphenyl degrading enzyme bphc from aquamicrobium sp. sk-2”. Journal of Japan Society of Civil Engineers, Ser G (Environmental Research). vol. 71, no. 7, pp.III_413- III_419, 2015.
[21] Nie Z.J., Hang B.J., Cai S., et al. “Degradation of cyhalofop-butyl (CyB) by Pseudomonas azotoformans strain QDZ-1 and cloning of a novel gene encoding CyB-hydrolyzing esterase”. Journal of Agricultural and Food Chemistry. vol. 59, no. 11, pp.6040-6046. 2011.

Subscription
E-Mail Alert
Most popular papers
Call for Special Issue
Publication Ethics & OA Statement
Plagiarism Policy
Contact Us
Downloads: 1334
Visits: 59704

Sponsors, Associates, and Links

All published work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright © 2016 - 2031 Clausius Scientific Press Inc. All Rights Reserved.