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Prognostic relationship between TIPIN and colorectal cancer and experimental validation

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DOI: 10.23977/medsc.2023.040717 | Downloads: 16 | Views: 193

Author(s)

Cao Yusheng 1, Tan Xijuan 1, Liang Haoyuan 1, Huang Tianfu 1, Huang Xusen 2

Affiliation(s)

1 Youjiang Medical University for Nationalities, Baise, Guangxi, China
2 Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, China

Corresponding Author

Huang Xusen

ABSTRACT

Colorectal cancer (CRC) is the third leading cause of death in the world, and there is no good treatment for it. The purpose of this experiment is to investigate the relationship between TIPIN and prognosis in colorectal cancer, as well as its effects on migration, proliferation, and epithelial-mesenchymal transition (EMT) in colorectal cancer. The expression of TIPIN in colorectal cancer was verified by Western blotting; the expression of E-calmodulin was analyzed by WB assay after transfection of TIPIN plasmid; the effects of TIPIN on the proliferation and migration of colorectal cancer cells were evaluated by cell scratch assay and CCK8 cell growth assay. High expression of TIPIN was closely related to patients' distant organ metastasis (P=0.018), lymph node invasion (P=0.038), significant correlation with tumor stage (P=0.024), no correlation was found with the diameter size of tumors, and patients with low expression of TIPIN had a better survival prognosis; at the same time, TIPIN has a close relationship with DNA damage and repair; TIPIN is highly expressed in colorectal cancer tissues, and high levels of TIPIN inhibit the expression level of E-calmodulin and promote the proliferation and migratory ability of colorectal cancer. TIPIN is associated with patient prognosis and affects proliferation, migration, and the EMT process in colorectal cancer.

KEYWORDS

Colorectal cancer; TIPIN; prognosis

CITE THIS PAPER

Cao Yusheng, Tan Xijuan, Liang Haoyuan, Huang Tianfu, Huang Xusen, Prognostic relationship between TIPIN and colorectal cancer and experimental validation. MEDS Clinical Medicine (2023) Vol. 4: 100-107. DOI: http://dx.doi.org/10.23977/medsc.2023.040717.

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