Author(s)

Miu Tianlin, Sunyanbin, Ren Guoli, Xue Chunmei

Corresponding Author

Xue Chunmei

ABSTRACT

To make a thorough inquiry about the optimum conditions for the separation of primary plastids from lavender-callus, providing basic data and theoretical basis for the optimization and breeding of lavender varieties. Using the lavender tender leaves as raw materials, the method of chemical induction was used to induce the dissociation of lavender to form the callus, which was used to dissociate the protoplast. The protoplast vitality was calculated with staining method. When cultured in MS medium with 2, 4-D (1.8mg/L) and 6-BA (0.5mg/L), lavender callus was the highest, up to 88%; the content of callus in cellulase and pectinase were 1.8% and 0.5% under the conditions of enzymatic hydrolysis yield of protoplasts the highest, up to 1.180×105 per gram, the highest protoplast viability, induction conditions can reach 78.05%. The efficient callus induced conditions of this experiment provide data support for further study of the subsequent callus regeneration system. The protoplast isolation system was established, and the protoplast of high yield and high vitality was obtained stably.

KEYWORDS

Lavender angustifolia, protoplast separation, tissue culture, protoplast preparation