Author(s)

Zebin Chen, Xi Gao, Li Lin, Yuan Su, Jiani Liu, Lei Yu, Dingkang Wang, Shengguang Xu

Corresponding Author

Shengguang Xu

ABSTRACT

In order to understand the composition of microorganisms in rhizosphere soil of tobacco plants, the genome DNA of 16S rDNA-V4 region and ITS1 region of the rhizosphere soil of healthy tobacco plants and plants infected by root-knot nematode were sequenced through high throughput sequencing technique; Qiime and Mothur software were used to analyze the abundance and diversity of the flora. The results show that, the OTU abundance and α diversity index of bacteria and fungi in the soil of healthy plants were higher than those of the infected plants. Bacteroidetes group bacteria, which was dominant in farmland ecosystem, only accounted for 2.12% and 1.81% in the rhizosphere soil of healthy and infected tobacco plants. The abundance of Actinobacteria group, which has biocontrol effects, was higher in the rhizosphere soil of healthy plants than that of infected tobacco plants. It suggests that, the low abundance of Bacteroidetes and Actinobacteria groups may serve as an indicator of root-knot nematode infection. The analysis of community composition shows that, the abundance of Ascomycota groups in the rhizosphere soil of infected tobacco plants was significantly lower than that of the healthy plants; the abundance of Basidiomycota groups in the rhizosphere soil of infected plants was higher than that of the healthy plants. It could be inferred that the abundance of these two types of bacteria was related to the occurrence of root-knot nematode infection.

KEYWORDS

High throughput sequencing, root-knot nematode, tobacco, rhizosphere soil microorganism