Author(s)

Li Lin, Bing Li, Zebin Chen, Yujiao Zhu, Yuan Su, Lei Yu, Shengguang Xu

Corresponding Author

Shengguang Xu

ABSTRACT

The aim of this research is to clarify the effects of different inoculated pathogens on the microbial diversity of rhizosphere soil of tobacco in marigold-tobacco intercropping system. The DNA of 16S rDNA-V4 region and ITS1 region in the rhizosphere soil of tobacco are sequenced with high throughput sequencer. The results show that after inoculating different pathogens, the abundances of Actinobacteria and Gemmatimonadetes increase; the abundance of Ascomycota decrease. In the tobacco-marigold intercropping system, the abundances of Actinobacteria, Gemmatimonadetes and Ascomycota increase. By inoculating different pathogens in tobacco plants in the marigold-tobacco intercropping system, and analyzing changes in microbial flora of the rhizosphere soil, this paper could provide a basis for analyzing the nematode killing mechanism of marigold.

KEYWORDS

Tobacco, Marigold, rhizosphere soil, high throughput sequencing, microbial diversity