Author(s)

Song Jin, Xi Gao, Zebin Chen, Yuan Su, Jiani Liu, Lei Yu, Dingkang Wang, Shengguang Xu

Corresponding Author

Shengguang Xu

ABSTRACT

In this experiment, the tobacco variety Yunyan 87 was cultivated by 3 different planting methods. Afterwards, tobacco leaves were taken as the research material. By using the high throughput sequencing technology, DNA in the 16S rDNA-V4 region of tobacco was sequenced to analyze the abundance and diversity of species, in order to understand the diversities of endophytic bacteria in tobacco under different kinds of planting methods. The results show that the sequence of micro flora richness of 3 tobacco samples was Y6.1 > Y7.1 > Y1.1; the sequence of bacteria diversity was Y7.1 > Y6.1 > Y1.1. The bacterial diversity of Y7.1 was higher than that of Y1.1 and Y6.1. Endophytic bacteria found in samples were distributed in 13 phyla, namely Proteobacteria, Bacteroidetes, Actinobacteria, Acidobacteria, Gemmatimonadetes, Firmicutes, Chloroflexi, Planctomycetes, Nitrospirae, Verrucomicrobia, Thaumarchaeota, Thermomicrobia and Armatimonadetes. Proteobacteria and Actinobacteria were dominant bacteria in Y1.1, which accounted for 61.56% and 18.26% of the total bacteria. Proteobacteria, Acidobacteria and Actinobacteria were dominant bacteria in Y6.1, which accounted for 47.42%, 19.71% and 13.40% of the total bacteria. Proteobacteria, Bacteroidetes and Actinobacteria were dominant bacteria of Y7.1, which accounted for 54.28%, 15.59% and 11.24% of the total bacteria. The distribution of Morganella endophytic bacteria in Y6.1 and Y7.1 was related to the addition of biological carbon. The distribution of Fusobacterium and Proteiniclasticum endophytic bacteria in Y7.1 was related to the inter cropping with marigold.

KEYWORDS

High throughput sequencing, tobacco, planting method, endophytic bacteria, diversity