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  4. Vol 3, Issue 1, 2025

The Toxic Effects of Polystyrene Microplastics on Colonic Epithelial NCM460 Cells

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DOI: 10.23977/medbm.2025.030106 | Downloads: 8 | Views: 407

Author(s)

Lizhuo Chen 1

Affiliation(s)

1 High School Affiliated to Renmin University of China, No. 1, South Hugezhuang Village, Lucheng Town, Tongzhou District, Beijing, China

Corresponding Author

Lizhuo Chen

ABSTRACT

Lots of recent studies focused on the impact of microplastics on human health and investigates the location of it inside the body. Most of the study aiming to orient the traces of polystyrene microplastic use a kind which pigments were added inside making it easier to be observed. But weather the treate particles could replace the original one? The following study was set to investigate the impact of original or marked polystyrene microplastic (PS MPs) on NCM460 cells and how the pigments within could have impact on them. Cells experienced a bath of different concentration(100μg/mL, 200μg/mL, 400μg/mL) of two different formulas including PS and colored PS. Cell Counting Kit - 8 (CCK - 8) assay was used to detect the cell viability. The result shows that, first, PS MPs do have an negative impact on the and the viability. On top of it, pigments also has significant impact on the result tested by the colored ones. CCK data decreased while ROS increased illustrating that oxidative stresses could also be triggered by PS MPs and the colored one. In a word, PS MPs do have an impact on human cells and colored ones increases the damage, as a result, colored ones shouldn't be seen as the substitute for the original PS MPs. This work provides theoretical foundations for the investigation of distribution within scientific studies.

KEYWORDS

Polystyrene microplastics; colored polystyrene microplastics; Intestinal cell; cytotoxicity; Reactive oxygen species; Oxidative stress

CITE THIS PAPER

Lizhuo Chen, The Toxic Effects of Polystyrene Microplastics on Colonic Epithelial NCM460 Cells. MEDS Basic Medicine (2025) Vol. 3: 32-36. DOI: http://dx.doi.org/10.23977/medbm.2025.030106.

REFERENCES

[1] Ramesh, Parkavi & Srinivas, Govindarajulu & Sundar, Jasmine & Kalpana, Sana & Srinivasan, Valarmathi. (2024). Easier To Use, Harder To Save The Environment: A Review On Plastic Pollution.
[2] Palaniappan, S., Sadacharan, C.M. & Rostama, B. Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro. Expo Health 14, 75–85 (2022). https://doi.org/10.1007/s12403-021-00419-3
[3] Cao J, Xu R, Wang F, et al. Polyethylene microplastics trigger cell apoptosis and inflammation via inducing oxidative stress and activation of the NLRP3 inflammasome in carp gills[J]. Fish & shellfish immunology, 2023, 132: 108470.
[4] Amobonye A, Bhagwat P, Raveendran S, et al. Environmental impacts of microplastics and nanoplastics: A current overview[J]. Frontiers in Microbiology, 2021, 12: 768297.
[5] Geyer R, Jambeck J R, Law K L. Production, use, and fate of all plastics ever made[J]. Science advances, 2017, 3(7): e1700782.
[6] Mahmud F, Sarker D B, Jocelyn J A, et al. Molecular and cellular effects of microplastics and nanoplastics: focus on inflammation and senescence[J]. Cells, 2024, 13(21): 1788.
[7] Wang L, Pei W, Li J, et al. Microplastics induced apoptosis in macrophages by promoting ROS generation and altering metabolic profiles[J]. Ecotoxicology and Environmental Safety, 2024, 271: 115970.
[8] Schröter L, Ventura N. Nanoplastic Toxicity: Insights and Challenges from Experimental Model Systems. Small. 2022 Aug;18(31):e2201680. doi: 10.1002/smll.202201680. Epub 2022 Jul 10. PMID: 35810458
[9] Hu R, Zhang K, Wang W, Wei L, Lai Y. Quantitative and sensitive analysis of polystyrene nanoplastics down to 50 nm by surface-enhanced Raman spectroscopy in water. J Hazard Mater. 2022 May 5;429: 128388. doi: 10.1016/j.jhazmat.2022.128388. Epub 2022 Jan 29. PMID: 35236023.
[10] Teng M, Zhao X, Wang C, et al. Polystyrene nanoplastics toxicity to zebrafish: dysregulation of the brain–intestine–microbiota axis[J]. ACS nano, 2022, 16(5): 8190-8204.
[11] Huang Z, Weng Y, Shen Q, et al. Microplastic: a potential threat to human and animal health by interfering with the intestinal barrier function and changing the intestinal microenvironment[J]. Science of the Total Environment, 2021, 785: 147365.
[12] Liang B, Zhong Y, Huang Y, et al. Underestimated health risks: polystyrene micro-and nanoplastics jointly induce intestinal barrier dysfunction by ROS-mediated epithelial cell apoptosis[J]. Particle and fibre toxicology, 2021, 18(1): 20. 
[13] Wang W, Zhang J, Qiu Z, et al. Effects of polyethylene microplastics on cell membranes: A combined study of experiments and molecular dynamics simulations[J]. Journal of Hazardous Materials, 2022, 429: 128323.
[14] Shirahama H, Suzawa T. Adsorption of bovine serum albumin onto styrene/acrylic acid copolymer latex[J]. Colloid and Polymer Science, 1985, 263: 141-146.

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