Author(s)

Christian Wang, Qinglang Liao, Yuxuan Xing

Corresponding Author

Christian Wang

ABSTRACT

Osteosarcoma is a common primary malignant bone tumor. At present, chemotherapy has certain toxic and side effects, thus it is of great significance to pursue new and effective alternative techniques. In this study, a novel drug-carrying magnetic targeting complex was prepared using magnetotactic bacterial magnetosomes as carrier, including doxorubicin (DOX), magnetotactic bacterial magnetosomes and plK1-shrNA eukaryotic expression plasmid with HSP70 heat shock promoter. The results of drug release tests showed that pHSP-PLK1-shRNA/DOX/BM compounds have a good in-body release potential without obvious instant releases condition. The proliferation activity of tumor cells was determined by MTT assay, the compound could inhibit the proliferation of osteosarcoma U2OS cells, especially under pulsed magnetic field exposure. The preparation of the complex provides a new possible clinical application approach and reference for magnetic targeted comprehensive therapy of osteosarcoma.

KEYWORDS

Osteosarcoma, Magnetotactic bacterial magnetosomes, Heat shock promoter, Cell proliferation inhibition