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  4. Vol 3, Issue 1, 2024

Colorimetric Detection of Alkaline Phosphatase (ALP) Based on Fe3O4@MIL@Pt

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DOI: 10.23977/analc.2024.030111 | Downloads: 23 | Views: 763

Author(s)

Bing Li 1

Affiliation(s)

1 School of Chemistry, South China Normal University, Guangzhou, Guangdong, China

Corresponding Author

Bing Li

ABSTRACT

This study presents an advanced colorimetric biosensor based on Fe3O4@MIL@Pt nanocomposites for the sensitive and selective quantification of alkaline phosphatase (ALP). The nanocomposite system combines the catalytic capabilities of Fe3O4 nanoparticles, the structural advantages of metal-organic frameworks (MIL), and the catalytic prowess of platinum (Pt) nanoparticles. Through the implementation of a triple signal amplification strategy, the catalytic efficiency of TMB oxidation is significantly boosted, resulting in a distinct blue color development of the oxidized TMB (OX-TMB). The assay is designed such that AA reduces OX-TMB, leading to a measurable decrease in absorbance, while ALP activity is reflected in the hydrolysis of ascorbic acid-2-phosphate (AA2P) to AA, thereby modulating the colorimetric signal. This system allows for highly sensitive detection of ALP by monitoring absorbance changes. The analytical performance is characterized by a detection limit of 0.091 mU/mL with a linear range from 0.5 to 10 mU/mL. Importantly, the method demonstrates excellent analytical performance with real serum samples, emphasizing its potential for clinical diagnostic applications.

KEYWORDS

Fe3O4@MIL@Pt; colorimetric method; 3,3',5,5'-Tetramethylbenzidine (TMB); ascorbic acid (AA); alkaline phosphatase (ALP)

CITE THIS PAPER

Bing Li, Colorimetric Detection of Alkaline Phosphatase (ALP) Based on Fe3O4@MIL@Pt. Analytical Chemistry: A Journal (2024) Vol. 3: 62-71. DOI: http://dx.doi.org/10.23977/analc.2024.030111.

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