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Intervention effects of Hellgrammites (Megaloptera: Corydalidae) on ‘Kidney-Yang Deficiency syndrome’ rats

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DOI: 10.23977/jasv.2018.11002 | Downloads: 52 | Views: 5795

Author(s)

Daifang Zhang 1, Yuyun Chen 1, Ming Pan 2

Affiliation(s)

1 Department of Chemical Engineering, Sichuan University of Science & Engineering, Zigong643000, Sichuan, China
2 School of of Bioengineering, Sichuan University of Science & Engineering, Zigong643000, Sichuan, China

Corresponding Author

Ming Pan

ABSTRACT

The present study was conducted to explore the effects of hellgrammites protein hydrocortisone administration induced Kidney-Yang Deficiency syndrome (KYDS) rats. The proteins were extracted from hellgrammites. Protein content was determined by Kjeldahl method. 51 rats were divided randomly into 5 groups. (group 1: blank control; group 2: KYDS model; group 3: KYDS rats treated with desmopressin(DDAVP); group 4: KYDS rats treated with hellgrammites protein low dose group 100 mg/kg; group 5: KYDS rats treated with hellgrammites protein high dose group 200 mg/kg). The rats were injected intraperitoneally hydrocortisone once daily for 18 days to simulate ‘Kidney-Yang Deficiency syndrome’, then the ninth day administered orally hellgrammites protein extract once daily for the following 12 days. The ALD, AVP, CAMP, CAMP/CGMP and renal tissue expression in different groups were compared. Compared with blank control diet group, CAMP/CGMP in hyperlipemia model group decreased ( P < 0. 05) and increased ALD(P >0.05). After treatment with hellgrammites protein high dose group and DDAVP group, CAMP/CGMP of the Kidney yang deficiency rats increased (P< 0. 05) and increased ALD(P<0. 05). The hellgrammites protein extract have protective effects on the rat model of kidney yang deficiency and polyuria caused by hydrocortisone.

KEYWORDS

Hellgrammite, Protein, Kidney-Yang Deficiency syndrome, ALD, CAMP

CITE THIS PAPER

Daifang, Z., Yuyun, C., Ming, P., Ling Y., Intervention effects of Hellgrammites(Megaloptera:Corydalidae) on ‘Kidney-Yang Deficiency syndrome’ rats. Journal of Animal Science and Veterinary (2018) 1: 6-16.

REFERENCES

[1] F. Gao, M. Lu, S.L. Yan, M.Q. Wang, W. He, Study of cluster analysis of symptom and sign differentiation’s factors of Kidney-Yang deficiency, Mod. J. Integr. Tradit. Chin. West. Med. 15 (2006) 2007–2009.
[2] Q. Chen, 1993. Research Methods in Pharmacology of Chinese Materia Medica, People’s Medical Publishing House, Beijing, pp. 982–984.
[3] Zhao, L., H. Wu, M. Qiu, W. Sun, R. Wei, X. Zheng, Y. Yang, X. Xin, H. Zou, T. Chen, J. Liu, L. Lu, J. Su, C. Ma, A. Zhao, and W. Jia. 2013. Metabolic Signatures of Kidney Yang Deficiency Syndrome and Protective Effects of Two Herbal Extracts in Rats Using GC/TOF MS. Evid Based Complement Alternat Med 2013:540957.
[4] Q. Chen, 1993. Experimental Methodology of Pharmacological Research in Traditional Chinese Medicine,People’sHealth PublishingHouse, Beijing, China.
[5] J.Yang, Y. Wang,Y.Bao,and J. Guo, 2008. “Tetotal flavonesfrom Semen cuscutae reverse the reduction of testosterone level and the expression of androgen receptor gene in kidney-yang deficient mice,” Journal of Ethnopharmacology,vol.119,no. 1, pp. 166–171.
[6] C.M.Wang, S. Y. Xu,S.Lai et al., “Curculigo orchioides (Xian Mao) modifies the activity and protein expression of CYP3A in normal and Kidney-Yang Deficiency model rats,” Journal of Ethnopharmacology,vol.144,no. 1, pp.33–38,2012.
[7] de Castro, R. J. S., A. Ohara, J. G. d. S. Aguilar, and M. A. F. Domingues. 2018. Nutritional, functional and biological properties of insect proteins: Processes for obtaining, consumption and future challenges. Trends in Food Science & Technology 76:82-89.
[8] Huang, D., J. Yang, X. Lu, Y. Deng, Z. Xiong, and F. Li. 2013. An integrated plasma and urinary metabonomic study using UHPLC-MS: intervention effects of Epimedium koreanum on 'Kidney-Yang Deficiency syndrome' rats. J Pharm Biomed Anal 76:200-206.
[9] Jepson, J. E., and S. W. Heads. 2016. Fossil Megaloptera (Insecta: Neuropterida) from the Lower Cretaceous Crato Formation of Brazil. Zootaxa 4098 (1):134-144.
[10] Rumpold, B. A., and O. K. Schluter. 2013. Nutritional composition and safety aspects of edible insects. Mol Nutr Food Res 57 (5):802-823.
[11] van Huis, A. 2013. Potential of insects as food and feed in assuring food security. Annu Rev Entomol 58:563-583.
[12] van Huis, A. 2016. Edible insects are the future? Proc Nutr Soc 75 (3):294-305.
[13] Xie, L., J. D. Hoffert, C. L. Chou, M. J. Yu, T. Pisitkun, M. A. Knepper, and R. A. Fenton. 2010. Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298 (4):F1018-1023.
[14] Cao, C. 2014. Situation of developing and utilizing crawling worm in china and species definition. Hubei Agricultural Sciences 21: 5061-5064.
[15] Yang, D., S. Tao, L. He, C. Wang and J. Li. 2012. Effects of protohermes grandis extract on reproductive capacity and life-span of drosophila melangogaste. Nat Prod Res Dev 24:366-369.
[16] Yang, D., J. Liang, P. Liao, C. Long, L. Luo and J. Li. 2014. Effects of protohermes grandis extract on growth and reproduction of mice. Nat Prod Res Dev 26:1290-1293.
[17] Deng HY, Gao Y, Ye H, Deng YM, Zhong F, Yang HB. 2008. Clinical observation of dry-bed training and Minirin treatment of children with primary enuresis. The Journal of Practical Medicine. 24(11):1928-1930. Chinese.
[18] Mo, JM., TW. Liu, RJ. Huang and J. Qian. 2013. Analysis of clinical features of primary aldosteronism in different sexes [J]. Chongqing Medicine 42(4):447-449.
[19] Chen X, Huang ZX. 2010. Expression and significance of AQPs in the injured kidney by N-hexane acute poisoning. Anatomy Research, 32(2): 110-114. 
[20] Maurice DH, Palmer D, Tilley DG, et al. 2003. Cyclic nucleotide phosphodiesterase activity, expression, and targeting in cells of the cardiovascular system[J]. Mol Pharmacol, (64):533-546.

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