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Application of Signal Molecule Enhanced ANAMMOX Process in Low Temperature Ammonia Nitrogen Wastewater Treatment

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DOI: 10.23977/erej.2020.040111 | Downloads: 10 | Views: 233


Peng Zhou 1, Shuo Zhang 1, Jun Li 1


1 Shenyang Jianzhu University, Hunnan East Road, Hunnan New District, Shenyang, Liaoning, China

Corresponding Author

Jun Li


As a new denitrification process, anammox has received extensive attention in the treatment of high ammonia nitrogen wastewater. At low temperature, ANAMMOX bacteria grew slowly and could not stay in the reactor. The reactor performance was poor. As a result, the process is greatly limited in engineering application. Appropriate addition of signal molecules can enhance the activity of ANAMMOX bacteria. The effect of the signal molecule 3-oxo-c8-hsl on the denitrification performance of anammox reactor running continuously at low temperature (15 ℃) was studied experimentally. Two parallel UASB reactors were used, one with 0.67ng/L of 3-oxo-c8-hsl signal molecule (R2) and the other without the addition of signal molecule as blank control (R1). During operation, R2 improved the maximum nitrogen removal rate by 25% compared with R1, and showed better load shock resistance. The batch experiment (SAA) showed that 3-oxo-c8-hsl could significantly improve the activity of anaerobic ammonia-oxidizing bacteria. In addition, 3-oxo-c8-hsl can maintain pH and REDOX potential (ORP) within the appropriate range, providing a stable environment for bacterial growth and reactor operation. The polysaccharides, proteins and total extracellular polymers (EPS) in the reactor increased by 5.4%, 35.2% and 29.7%, respectively, over the original sludge. Particle size and scanning electron microscope (SEM) showed that the sludge particles were compact and particle size was smaller. The addition of signal molecules enhances the reaction performance of ANAMMOX at low temperature, which can promote the application of ANAMMOX process in sewage plants in cold areas.


Anammox, N-removal, signal molecule, low temperature, wastewater treatment


Peng Zhou, Shuo Zhang, Jun Li, Application of Signal Molecule Enhanced ANAMMOX Process in Low Temperature Ammonia Nitrogen Wastewater Treatment. Environment, Resource and Ecology Journal (2020) 4: 70-80. DOI:


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