Based on the Analysis of 16S rDNA Sequence, the Change Rule of Microorganism in the Process of Processing Jianchang Banya was proposed
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DOI: 10.23977/ESAC2020011
Author(s)
Li Cai, Qiao Lin, Xubin Hua, Liuwui Yang, Guochao Gan
Corresponding Author
Qiao Lin
ABSTRACT
In the whole fermentation process of Jianchang salted duck, the change of microbial quantity, species and dominant population in different periods were studied. Seven different culture media were used to isolate and purify all kinds of microorganisms, and 16S rDNA sequencing method was used to identify the microorganisms. The results showed that the dominant populations were lactobacillus, yeast and glucose bacteria. In this study, the DNA of the bacterial strain was analyzed by the OUT analysis method, and the corresponding classification information of each species was obtained. The results showed that among the three dominant populations, the main bacterial colonies were lactobacillus acidophilus, pichia Pasteur yeast and staphylococcus rimidis, which had a relatively large impact on the quality and flavor of the products.
KEYWORDS
Jianchang control; rule of microbial change; dominant population; 16s rDNA; OUT analysis