Author(s)

Zhao Hang ¹, Miao Yining ¹, Yang Jun ²

Affiliation(s)

¹ College of Life Science, Zhejiang Normal University, Jinhua, Zhejiang, China
² School of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, Zhejiang, China

Corresponding Author

Zhao Hang

ABSTRACT

In order to alleviate the adverse effects of soil salinity and cadmium pollution on the quality of tomato production, the present study was conducted to investigate the effects of SA on the physiological response and DNA damage of tomato under different salt and cadmium stress conditions in the control, cadmium, salt and cadmium groups, using the "Cooperative 908" tomato as the test material, with the control, cadmium, salt and cadmium groups set up, and with the application of a series of exogenous SA solutions in a series of concentration gradient, at 7d, 14d, and 21d. The effects of SA on the physiological response and DNA damage of tomato under different salt and cadmium stress conditions were investigated after 7d, 14d and 21d. The results showed that a certain concentration of salt and cadmium stress would inhibit the normal growth and development of tomato, causing damage to the membrane system, photosynthetic system and DNA damage in the root system. And the application of SA can effectively reduce the symptoms of tomato poisoning: plant height, root length and leaf area increased; antioxidant enzyme activities increased significantly, and the maximum increase of POD, SOD and CAT activities under double stress was 90.22%, 4.78% and 40.30%, respectively; the decrease of MDA content was 49.01%; and the maximum growth rates of proline content, chlorophyll content were 168.60% and 32.69%, respectively; fluorescence parameters were significantly improved; and the degree of DNA trailing was significantly reduced. In conclusion, the application of exogenous SA can effectively enhance the resistance of tomato under salt and cadmium stress and promote the good growth of tomato, which lays the foundation for further exploring the application of SA in agriculture.

KEYWORDS

Salicylic acid; salt stress; cadmium stress; tomato; physiological response; DNA damage

CITE THIS PAPER

Zhao Hang, Miao Yining, Yang Jun, Effects of exogenous salicylic acid on physiological response and DNA damage in tomato under double stress of salt and cadmium. Journal of Modern Crop Science (2024) Vol. 3: 25-36. DOI: http://dx.doi.org/10.23977/jmcs.2024.030104.

REFERENCES

[1] Huang Y, Wang L, Wang W, et al. Current status of agricultural soil pollution by heavy metals in China: a meta-analysis[J]. Science of the Total Environment, 2019, 651(2):3034-3042.
[2] Manasa M, Katukuri N, Nair S, et al. Role of biochar and organic substrates in enhancing the functional characteristics and microbial community in a saline soil[J]. Journal of Environmental Management, 2020, 269(3): 110737.
[3] Parihar P, Singh S, Singh R, et al. Effect of salinity stress on plants and its tolerance strategies: a review[J]. Environmental Science and Pollution Research International, 2015, 22(6):4056-4075.
[4] Chen N C, Zheng Y J, He X F, et al. Analysis of the bulletin of national soil pollution survey[J]. Journal of Agro-Environmental Science, 2017, 36(9):1689-1692.
[5] Dalcorso G, Farinati S, Maistri S, et al. How plants cope with cadmium: Staking all on metabolism and gene expression [J]. Journal of Integrative Plant Biology, 2008, 50(10):1268-1280.
[6] Gong Z Z, Xiong L M, Shi H Z, et al. Plant abiotic stress response and nutrient use efficiency[J]. Science China Life Sciences, 2020, 63(5):635-647.
[7] Gatsios A, Ntatsi G, Yfantopoulos D, et al. Effects of different organic soil amendments on nitrogen nutrition and yield of organic greenhouse tomato crop[J]. Nitrogen, 2021, 2(3):347-358.
[8] Luo Yan. Effects of exogenous salicylic acid and melatonin on physiological characteristics and antioxidant enzyme gene expression in tomato seedlings under drought stress[D]. Nanning: Guangxi University, 2022.
[9] WANG Tongming, CHEN Wei, PAN Wenjie, et al. Effects of organic and chemical fertilisers on gas exchange, chlorophyll fluorescence characteristics and chloroplast ultrastructure of tobacco leaves[J]. Journal of Plant Nutrition and Fertiliser, 2015, 21(2):517-526.
[10] XIAO Shuang, HAN Yuchen, NO Yu Ran, et al. Effects of polyethylene glycol initiation on germination and physiological characteristics of cotton seeds under salt stress[J]. Journal of Nuclear Agriculture, 2021, 35(1):202-210.
[11] Jday A, Rejeb K, Slama I, et al. Effects of exogenous nitric oxide on growth, proline accumulation and antioxidant capacity in Cakile maritima seedlings subjected to water deficit stress[J]. Functional Plant Biology, 2016, 43(10):939-948.
[12] CHEN Lu, ZHANG Xiaoli, GAO Zhu, et al. Effects of lanthanum nitrate spraying on osmoregulatory substances in navel orange leaves[J]. Chinese Agronomy Bulletin, 2021, 37(29):114-119.
[13] Cma B, Hgvf A, Gp B, et al. Effects of water and nutrient availability on morphological, physiological, and biochemical traits of one invasive and one native grass of a Neotropical savanna[J]. Environmental and Experimental Botany, 2020, 182(1):104305.
[14] Møller P, Azqueta A, Boutet-Robinet E, et al. Minimum information for reporting on the comet assay (MIRCA): recommendations for describing comet assay procedures and results[J]. Nature Protocols, 2020, 15(12):3817-3826.
[15] WU Mengdan, YE Xu, WEI Dayong, et al. Physiological effects of growth regulators in alleviating salt stress in cherry radish[J]. Jiangxi Journal of Agriculture, 2023, 35(2):89-95.
[16] JIANG Haoliang, HUANG Yun, LIANG Shaofang, et al. Effects of cadmium stress on seedling growth of different sweet corn inbred lines and their related simple repeat molecular markers[J]. Zhejiang Journal of Agriculture, 2022, 34(8):1582-1590. 
[17] Afrin S, Akhtar N, Khanam T, et al. Alleviative effects of zinc on biomass yield and antioxidative enzymes activity in leaves of soybean (Glycine max L.) under salt stress[J]. American Journal of Agriculture and Forestry, 2021, 9(3):147-155.
[18] Muthuraja R, Muthukumar T. Co-inoculation of halotolerant potassium solubilising Bacillus licheniformis and Aspergillus violaceofuscus improves tomato growth and potassium uptake in different soil types under salinity[J]. Chemosphere, 2022, 294(5):133718.
[19] SU Shiping, LI Yi, LIU Xiaoe, et al. Mechanism of exogenous proline in alleviating drought stress in red sand[J]. Journal of Grass Industry, 2022, 31(6):127-138.
[20] Jung H I, Lee B R, Chae M J ,et al. Sulfur alleviates cadmium toxicity in rice (Oryza sativa L.) seedlings by altering antioxidant levels[J]. Journal of Crop Science & Biotechnology, 2017, 20(3):213-220.
[21] Yu K S, Bhandari S R, Jo J S, et al. Effect of drought stress on chlorophyll fluorescence parameters, phytochemical contents, and antioxidant activities in lettuce seedlings[J]. Horticulturae, 2021, 7(8):238.
[22] Pang Jinping, Wang Yongsheng. Response of photosynthetic and chlorophyll fluorescence parameters of oilseed rape seedlings to drought stress and analysis of their drought resistance[J]. Northwest Journal of Botany, 2023, 43(2):276-284.
[23] AI Jinxiang, SONG Jiayi, YAN Zhennan, et al. Regulatory effects of melatonin on the physiological responses and DNA damage of Rhizophora tigris and Rhizophora verticillata under lead stress[J]. Journal of Botany, 2022, 57(2):171-181.
[24] ZHOU Xuan, SHIN Lu, JIN Yuan, et al. Effects of exogenous salicylic acid on growth and major physiological characteristics of tea tree under salt stress[J]. Journal of Northwest Agriculture and Forestry University (Natural Science Edition), 2015, 43(7):161-167.
[25] Duan Wenjing, Meng Yanjun, Jiang Dan, et al. Effects of exogenous melatonin on morphology and antioxidant system of cotton seedlings under salt stress[J]. Chinese Journal of Ecological Agriculture, 2022, 30(1):92-104.
[26] HE Guoqiang, LIU Xi, GUO Zhennan, et al. Effects of cadmium stress on photosynthetic and chlorophyll fluorescence characteristics of roasted tobacco leaves[J]. North China Journal of Agriculture, 2016, 31(S1):388-393.
[27] ZHANG Ya, SHI Shuqian, LI Yaping, et al. Osmotic adjustment and chlorophyll fluorescence properties of wheat leaves under different salt stresses[J]. Journal of Applied Ecology, 2021, 32(12):4381-4390.
[28] Liu B, Zhao S, Tan F, et al. Changes in ROS production and antioxidant capacity during tuber sprouting in potato[J]. Food Chemistry, 2017, 237(15):205-213.
[29] Al-Mansouri M A, Ali I M. Effect of hydrogen peroxide and zinc spraying on the growth, yield and antioxidant enzyme of wheat Triticum aestivum L.[J]. Earth and Environmental Science, 2021, 904(1):012071.
[30] Marzena S R. Reactive oxygen species production and antioxidative defence in pea (Pisum sativum L.) root nodules after short-term aluminium treatment[J ]. Acta Physiologiae Plantarum, 2012, 34(4):1387-1400.
[31] MA Yonghui, LI Yongjie, LI Jin. Physiological response of black-fruited wolfberry seedlings to exogenous salicylic acid under dual stress of NaCl and drought[J]. Guangxi Plant, 2022, 42(4):668-675.
[32] Angelé-Martínez C, Goodman C, Brumaghim J. Metal-mediated DNA damage and cell death: mechanisms, detection methods, and cellular consequences[J]. Metallomics:integrated biometal science, 2014, 6(8):1358-1381.
[33] CHEN Suyu, ZHAO Qiang, et al. Analysis of DNA damage in root system of soybean seedlings by salt stress based on RAPD[J]. Journal of Ecology, 2022, 41(7):1441-1447.

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